Library Dissertation Showcase

Characterisation of the interaction between Bromodomain-Containing 1 Protein (BRD1) and Replication Protein A (RPA) in the homologous recombination pathway as targets for combinational therapy of breast cancer

  • Year of Publication:
  • 2020

Breast cancer affects around 2.1 million people globally each year. With a mortality rate around 30%, research into chemotherapeutics for chemotherapy, one of the most used treatments, is essential. Chemotherapy not only aims to damage cancerous cells’ DNA to the point of cell death, but also seeks to inhibit DNA repair pathways. One such pathway is the homologous recombination pathway and previous research into screening for novel players identified the Bromodomain-Containing 1 protein (BRD1). This study was designed to further investigate BRD1’s involvement in homologous recombination by analysing the potential protein-protein interaction with Replication Protein A (RPA). Through GFP-Nanotrapping of a generated GFP-tagged RPA cell line and BRD1/RPA knockdown, both results highlighted a potential interaction. This was shown through the presence of BRD1 proteins in the bead eluate of the GFP-Nanotrap, as well as through confocal microscopy analysis of co-localisation foci for BRD1 and RPA following camptothecin treatment. This study furthers the knowledge of the possible BRD1-RPA interaction in homologous recombination and cements the foundations for further research into this protein-protein interaction.

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